Ricerc@Sapienza

DECODE - High Density Multi Electrode Array

BioCAM DupleX is a state-of-the-art high-resolution microelectrode array platform for powerful in vitro functional imaging. This advanced platform works with CMOS-based MEA chips (HD-MEAs) able to acquire signals from thousands of electrodes simultaneously at high sampling frequency (20 kHz) to monitor both: slow large-population field potentials and fast single-cell spiking activity. The platform integrates reading, stimulator, and temperature control modules in a small footprint (230 mm/180 mm/43 mm; w/d/h) for an easy integration with instruments like microscopes, perfusion or patch-clamp systems. HD-MEAs provide a 3.8 mm x 3.8 mm grid of 4096 electrodes, each of them is 21 µm x 21 µm, with a 60 µm pitch. All these features allow full monitoring of electrophysiological signals from large tissue portions with a cellular resolution. Moreover, the BioCAM DupleX can use any of the recording sites to release an electrical stimulation, thus making it a fully bidirectional platform. This advanced platform works with both HD-MEAs with planar electrodes as well as the latest generation (Khíron Chips) which features 4096 ¿Needle electrodes. 3D electrodes can penetrate structured neuronal tissues obtaining signals from the inner layers of biological models such as brain slices, organoids, or spheroids. This revolutionary technology allows the full exploitation of the use of neuronal 3D models in applications such as drug discovery, toxicology, personalized medicine. The BioCAM DupleX is the unique Microelectrode Array platform of its kind in the market, able to power 3D high-density electrodes arrays. BioCAM DupleX is coupled with BrainWave, a unique integrated software for recording, stimulating and real-time signal processing. Hardware and software are designed to study neuronal functional connectivity and to decipher complex neuronal network behaviors. The locking mechanism of the BioCAM DupleX is equipped with a motorized system activated by the simple press of a button that will guarantee an always perfect contact alignment with the chip. A set of LEDs shows the status of the system indicating if it is powered, a chip is locked, or if a recording is running. BioCAM DupleX is compatible with all the 3Brain¿s MEA probe series, comprising the 1st and 2nd generation (Prime, Arena and Stimulo chips) and the latest family Khíron with the fully-bidirectional Accura and Accura 3D probes. Other technical specifications: REAL-TIME PROCESSING: Advanced FPGA control to denoise, filter and compress high-density data. MAGNETIC SURFACES: All the surfaces around the MEA chip are magnetic for easy setup with other equipment, such as perfusion holders. TEMPERATURE CONTROL: Integrated heating and cooling system. INTEGRATED STIMULATOR: 2 independently programmable stimulator channels (1 voltage and 1 current). FAST LOCKING SYSTEM: A motorized solution to reliably lock and unlock your microelectrode array (MEA) chips. ROBUST EXTERNAL CASE: Crafted from aluminum to shield from electromagnetic and mechanical noise.