brain development | Ricerc@Sapienza

Patch clamp recording and fluorescence monitoring system for brain cells and slices- MicroSynRec

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the integrated electrophysiology/fluorescence system allows the concomitant monitoring of membrane currents and fluorescence in brain cellsform acute brain slices.

The laboratory is optimized for patch clamp recording of evoked and spontaneous synaptic activity. moreover, it allows the rapid application of exogenous subtances. fluorescence monitoring allows the analysis of cells and cell processes movement in slices

Apparent diffusion coefficient assessment of brain development in normal fetuses and ventriculomegaly

Diffusion neuro-MRI has benefited significantly from sophisticated pre-processing procedures aimed at improving image quality and diagnostic. In this work, diffusion-weighted imaging (DWI) was used with artifact correction and the apparent diffusion coefficient (ADC) was quantified to investigate fetal brain development. The DWI protocol was designed in order to limit the acquisition time and to estimate ADC without perfusion bias. The ADC in normal fetal brains was compared to cases with isolated ventriculomegaly (VM), a common fetal disease whose DWI studies are still scarce.

Insulin resistance, oxidative stress and mitochondrial defects in Ts65dn mice brain: a harmful synergistic path in down syndrome

Dysregulation of brain insulin signaling with reduced downstream neuronal survival and plasticity mechanisms are fundamental abnormalities observed in Alzheimer disease (AD). This phenomenon, known as brain insulin resistance, is associated with poor cognitive performance and is driven by the inhibition of IRS1. Since Down syndrome (DS) and AD neuropathology share many common features, we investigated metabolic aspects of neurodegeneration in DS and whether they contribute to early onset AD in DS.