Unusual complexation behavior between daclatasvir and γ-Cyclodextrin. A multiplatform study
During a screening of cyclodextrins (CDs) as chiral selectors for the separation of daclatasvir (DCV) and its enantiomer by capillary electrophoresis (CE), an unusual phenomenon for CDs was observed, that is two peaks with a plateau in between using γ-CD as chiral selector. The same result was encountered when enantiopure DCV was injected or when analyzing a sample containing enantiopure DCV and γ- CD in a CD-free background electrolyte. Peak coalescence was observed at 45 °C and at a pH above 3.5.