brain slices | Ricerc@Sapienza

Patch clamp recording and fluorescence monitoring system for brain cells and slices- MicroSynRec

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the integrated electrophysiology/fluorescence system allows the concomitant monitoring of membrane currents and fluorescence in brain cellsform acute brain slices.

The laboratory is optimized for patch clamp recording of evoked and spontaneous synaptic activity. moreover, it allows the rapid application of exogenous subtances. fluorescence monitoring allows the analysis of cells and cell processes movement in slices

Preparation of rat hippocampal organotypic cultures and application to study amyloid beta-peptide toxicity

Hippocampal organotypic cultures constitute a very easy but delicate method widely used to study amyloid β-peptide toxicity. This ex vivo technique is performed on tissues isolated from newborn rats. Here, we describe a protocol for the preparation and culture of hippocampal organotypic slices that can be maintained for 14-21 days and their application to the study of amyloid β-peptide toxicity.