Profact - Protein Factory Training Lab - is a research lab (BSL1) for medium throughput production of biomass for recombinant protein expression.
ProFact-Lab is hosted at the Department of Biochemical Sciences “A. Rossi Fanelli” (CU027), known for its long-lasting experience on recombinant protein production and characterization.
Animals respond to chemical stress with an array of gene families and pathways termed “chemical defensome”. In arthropods, despite many defensome genes have been detected, how their activation is arranged during toxic exposure remains poorly understood. Here, we sequenced the transcriptome of Anopheles stephensi larvae exposed for six, 24 and 48?hours to the LD50 dose of the insecticide permethrin to monitor transcriptional changes of defensome genes across time.
The newly synthesized coumarin derivative with dopamine, 3-(1-((3,4-dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione, was completely structurally characterized by X-ray crystallography. It was shown that several types of hydrogen bonds are present, which additionally stabilize the structure. The compound was tested in vitro against different cell lines, healthy human keratinocyte HaCaT, cervical squamous cell carcinoma SiHa, breast carcinoma MCF7, and hepatocellular carcinoma HepG2.
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