Ricerc@Sapienza

Dierent acellular assays were developed to measure particulate matter’s (PM) oxidative potential (OP), a metric used to predict the ability of PM in generating oxidative stress in living organisms. However, there are still fundamental open issues regarding the complex redox equilibria among the involved species which could include reducing compounds. The aim of this study was the pilot application of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to PM in order to evaluate the presence of reducing species.

Different acellular assays were developed to measure particulate matter’s (PM) oxidative potential (OP) to predict the PM ability to generate oxidative stress in living cells and organisms. However, there are still fundamental unresolved issues regarding the influence of assay’s design and the tests’ operative condition on OP obtained results. An additional important point is related to the stability of the species: short-life oxidant species can react and redox equilibria among PM native species could occur during both the sample storage and the extraction phase.