Searching for inhibitors of human H 2 S-synthesizing enzymes by orthogonal methods
Hydrogen sulfide (H2S), a key signalling molecule in human patho-physiology, is produced by the human enzymes cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (MST). Increasing evidence indicates a correlation between increased H2S production and human diseases, such as several cancer types and amyotrophic lateral sclerosis. Identifying selective and potent inhibitors may be beneficial for future therapeutic strategies.