Association of exposure to benzene and smoking with oxidative damage to nucleic acids by means of biological monitoring of general population volunteers
In this study, the validation of liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) isotopic dilution method for the determination of benzene and nicotine metabolites in urine was carried out. Limit of detection are 0.026 ?g/L for S-phenylmercapturic acid (SPMA), 0.55 ?g/L for t,t-muconic acid (t,t-MA), and 12.41 ?g/L for the cotinine, and the relative combined uncertainty was also calculated. The study involves 446 healthy volunteer residents since at least 10 years in an area of central Italy.