Anabolic steroids

Development and applications of analytical procedures for the analysis of sulphate metabolites of anabolic androgenic steroids

The World Anti-Doping Agency (WADA) includes anabolic androgenic steroids (AAS) among the substances
and methods prohibited in sports. The process of steroids detection undertaken by the WADA accredited
laboratories is based on GC-MS(/MS) or LC-MS(/MS) analysis of urinary samples collected from male and
female athletes. The methods routinely used in steroid screening mainly focus on substances that are excreted
unconjugated or as glucuro-conjugates and for this reason common procedures includes enzymatic hydrolysis

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