Optimization and application of the DPPH assay for evaluating reducing properties of particulate matter
Different acellular assays were recently developed to measure particulate matter (PM) oxidative potential (OP) [1]. Common OP methods include dithiothreitol assay (OPDTT), ascorbic acid assay (OPAA) and 2?,7?-dichlorofluorescin (OPDCFH). OP assays can provide observations on the relationship between PM characteristics and its ability to generate oxidative stress. However, along with oxidizing species, experimental OP values suggest the possible presence of reducing species in PM. Therefore, the obtained data drives the need to deepen knowledge on redox properties of PM.