Ricerc@Sapienza

Sea bass represents one of the main fish products in the market. Most of it comes from farming and is bred in different conditons with respect to the wild fish. Differences may thus be expected. In this study, a proteomic profile of farmed and wild sea bass samples was performed, employing a fractionation strategy where peptide samples were first separated by 2D chromatography. The peptides were finally analyzed by shotgun proteomics workflow combined to tandem MS.

A rapid and selective method for the determination of phosphatidylcholine (PC) is described. It is based on the use of a single packed bed reactor in which phospholipase C, alkaline phosphatase and choline oxidase are co-immobilized on long chain-alkylamino controlled pore glass. The detection of hydrogen peroxide, that is the final product of subsequent enzymatic reactions occurring within the bioreactor, is used for the determination of PC in a dietary supplement. The results of triplicate analysis show a coefficient of variation of 5%.

Background: Phosphodiesterases (PDE) are a superfamily of enzymes that hydrolyse cyclic nucleotides (cAMP/
cGMP), signal molecules in transduction pathways regulating crucial aspects of cell life. PDEs regulate the intensity
and duration of the cyclic nucleotides signal modulating the downstream biological efect. Due to this critical role
associated with the extensive distribution and multiplicity of isozymes, the 11 mammalian families (PDE1 to PDE11)