Ricerc@Sapienza

This work presents a bio-microsystem, based on thin film optoelectronic devices, for on-chip detection of fluorescence-based aptamer assay. The detection is achieved integrating on a single glass substrate amorphous silicon photosensors and a thin film interferential filter, while the aptamer-based biomolecular recognition is carried out in a microfluidic network functionalized with polymer brushes. To test the developed platform, aptamer 1.12.2 having high affinity toward Ochratoxin A molecules has been used in combination with the ruthenium complex [Ru(phen)2(dppz)]2+ intercalating dye.

This paper reports on the development of a fluorescent label-free aptamer assay integrated in a lab-on-chip (LoC) system for the detection of Ochratoxin A (OTA). The detection system relies on the integration, on a single glass substrate, of an array of amorphous silicon photosensors and a long pass interferential filter. The aptamer assay, integrated into the microfluidic network, is an aptasensor having affinity versus OTA, selected as a case study. The fluorescent molecule is a "light switch" complex [Ru(phen)2(dppz)]2+.