This paper describes the development of an original micro-solid phase extraction device and its evaluation for the isolation of F2-isoprostanes (F2-IsoPs) from cord and maternal plasma samples. The unit is very simple and consists in a rotating disc (1.8 cm diameter) of oxidized buckypaper (BP), enwrapped in a polypropylene mesh pouch.
This chapter offers a concise overview on conventional methods and on the latest advancements in liquid chromatography analysis of water-soluble and fat-soluble vitamins. Since there are a significant variety of biologically active forms and matrices of interest, some recent techniques, instruments and approaches have been considered due to their importance in solving complex separations of vitamin homologues as well as comprehensive profiling of organic micronutrients in biological samples.
The possibility of preserving human tissue separated from the body, from which to extract clinical information, even on large numbers of individuals with similar clinical conditions, represents a great opportunity for the progress of biomedicine.The collection of biological materials in compliance with ethical and legal standards could be seen as the main innovation of research biobanks. Firstly, collections of samples are virtually unusable today because of the lack of detail in the informed consent or because they were catalogued following inappropriate methods.
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