Caco-2 cells | Ricerc@Sapienza

Proteomic analysis and bioluminescent reporter gene assays to investigate effects of simulated microgravity on Caco-2 cells

Microgravity is one of the most important features in space?ight. Previous evidence from in-vitro studies has shown that signi?cant changes occur under simulated microgravity. For thisreason, human colon adenocarcinoma Caco-2 cells were selected as cell model of intestinalepithelial barrier and their response to altered gravity conditions was investigated, especially onthe protein level.

The foodborne strain Lactobacillus fermentum MBC2 triggers pept-1-dependent pro-longevity effects in Caenorhabditis elegans

Lactic acid bacteria (LAB) are involved in several food fermentations and many of them
provide strain-specific health benefits. Herein, the probiotic potential of the foodborne strain
Lactobacillus fermentum MBC2 was investigated through in vitro and in vivo approaches.
Caenorhabditis elegans was used as an in vivo model to analyze pro-longevity and anti-aging effects.
L. fermentum MBC2 showed a high gut colonization capability compared to E. coli OP50 (OP50) or
L. rhamnosus GG (LGG). Moreover, analysis of pumping rate, lipofuscin accumulation, and body