Probing the interaction interface of the GADD45?/MKK7 and MKK7/DTP3 complexes by chemical cross-linking mass spectrometry
GADD45? is selectively and constitutively expressed in Multiple Myeloma cells, and this expression correlates with an unfavourable clinical outcome. GADD45? physically interacts with the JNK kinase, MKK7, inhibiting its activity to enable the survival of cancer cells. DTP3 is a small peptide inhibitor of the GADD45?/MKK7 complex and is able to restore MKK7/JNK activation, thereby promoting selective cell death of GADD45?-overexpressing cancer cells.