Non-aqueous reversed-phase liquid-chromatography of tocopherols and tocotrienols and their mass spectrometric quantification in pecan nuts
An easy and effective analytical method was developed for the simultaneous quantification of four tocopherols
(Ts) and four tocotrienols (T3s) in three pecan nut cultivars (Stuart, Sioux, and Pawnee). The analytes were
separated on a C30 column kept at 15 °C, under isocratic non-aqueous reversed-phase (NARP) conditions, in only
18 min and detected by atmospheric pressure chemical ionization–tandem mass spectrometry (APCI-MS/MS).
The HPLC-APCI-MS/MS method was validated, according to the main FDA guidelines, and then applied for the