In vivo bioluminescence-based monitoring of liver metastases from colorectal cancer: An experimental model
In this study we aimed to develop a new in vivo bioluminescence-based tool to monitor and to quantify colon cancer (CC) liver metastasis development. HCT 116 cells were transducted with pLenti6/V5-DEST- fLuc for constitutive expression of firefly luciferase. Infection was monitored analyzing endogenous bioluminescence using the IVIS Lumina II in vivo Imaging System and a positive clone constitutively expressing luciferase (HCT 116-fLuc) was isolated. HCT 116-fLuc cells were left untreated or treated with 1 M GDC-0449, a Hedgehog pharmacological inhibitor.