composting plant

Development of an analytical method for the metaproteomic investigation of bioaerosol from work environments

The metaproteomic analysis of air particulate matter provides valuable information about the properties of bioaerosols in the atmosphere and their influence on climate and public health. In this work, a new method for the extraction and analysis of proteins in airborne particulate matter from quartz microfiber filters was developed. Different protein extraction procedures were tested to select the best extraction protocol based on protein recovery.

Concentrations of bacteria and bacterial and fungal spores calculated from chemical tracers associated with size-segregated aerosol in a composting plant

The lack of information on biological risks in workplaces arises from the difficulty to measure bioaerosol. This study aimed to develop and improve the bioaerosol monitoring technique that uses proper biomarkers as a tool. Muramic and dipicolinic acids, and ergosterol were used as tracers for bacteria cells, bacterial spores, and fungal spores, respectively. Furthermore, 12- and 13- methyltetradecanoic acids (iso- and anteiso- C15:0) were used to study the presence of airborne bacteria and 3-hydroxy fatty acids were used to determine the concentration of peptidoglycan.

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