Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry
Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 ?m sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions.