Ricerc@Sapienza

The immobilization procedure is one of the key factors affecting the electron transfer based biosensors performance. It must assure both the enzyme retention activity and an efficient communication between the redox center of the enzyme and the electrode surface. In this regard, hydrogels are attractive materials due to their three-dimensional hydrophilic networks and their high-water concentration, promoting the biomolecule long-term stability and providing a suitable scaffold for trapping.