Stable oxidative cytosine modifications accumulate in cardiac mesenchymal cells from Type2 ddiabetes patients: Rescue by alpha-ketoglutarate and TET-TDG functional reactivation
RATIONALE: Human cardiac mesenchymal cells (CMSCs) are a therapeutically relevant primary cell population. Diabetes mellitus compromises CMSC function as consequence of metabolic alterations and incorporation of stable epigenetic changes.
OBJECTIVE: To investigate the role of α-ketoglutarate (αKG) in the epimetabolic control of DNA demethylation in CMSCs.