The "ChemiDoc MP" is a very flexible imaging system, capable of detecting colorimetric, chemiluminescent, fluorescent, red, green, blue and UV signals. It is also compatible with the 48 “Stain Free” technology for the visualization of proteins, immediately after the electrophoretic run.
Pectic homogalacturonan (HG) is one of the main constituents of plant cell walls. When processed to low degrees of esterification, HG can form complexes with divalent calcium ions. These macromolecular structures (also called egg boxes) play an important role in determining cell wall biomechanics and in mediating cell-to-cell adhesion. Current immunological methods enable only steady-state detection of egg box formation in situ. Here we present a tool for efficient real-time visualisation of available sites for HG crosslinking within cell wall microdomains.
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