Ricerc@Sapienza

The recent years witnessed a change in the perception of nutrition. Diet does not only provide nutrients to meet the metabolic requirements of the body, but it also constitutes an active way for the consumption of compounds beneficial for human health. Fruit and vegetables are an excellent source of such compounds, thus the growing interest in characterizing phytochemical sources, structures and activities.

An easy and effective analytical method was developed for the simultaneous quantification of four tocopherols
(Ts) and four tocotrienols (T3s) in three pecan nut cultivars (Stuart, Sioux, and Pawnee). The analytes were
separated on a C30 column kept at 15 °C, under isocratic non-aqueous reversed-phase (NARP) conditions, in only
18 min and detected by atmospheric pressure chemical ionization–tandem mass spectrometry (APCI-MS/MS).
The HPLC-APCI-MS/MS method was validated, according to the main FDA guidelines, and then applied for the

It is well established that different factors affect milk composition in cows and that milk composition, in turn, affect both technological and nutritional qualities. In this respect the comprehension of the metabolic variability of milk composition in relation to the lactation time as well as to the genetic background may be of paramount importance for the agri-food industries. In the present study we investigated the variations of the metabolic profiles during lactation in milks obtained from Friesian and autochthonous races from Northern Italy by 1H NMR metabolomics.