fructose dehydrogenase | Ricerc@Sapienza

Direct electron transfer of dehydrogenases for development of third generation biosensors and enzymatic fuel cells

Dehydrogenase based bioelectrocatalysis has been increasingly exploited in recent years in order to develop new bioelectrochemical devices, such as biosensors and biofuel cells, with improved performances. In some cases, dehydrogeases are able to directly exchange electrons with an appropriately designed electrode surface, without the need for an added redox mediator, allowing bioelectrocatalysis based on a direct electron transfer process.

Enhanced direct electron transfer of fructose dehydrogenase rationally immobilized on a 2-aminoanthracene diazonium cation grafted single-walled carbon nanotube based electrode”

In this paper, an efficient direct electron transfer (DET) reaction was achieved between fructose dehydrogenase (FDH) and a glassy carbon electrode (GCE) onto which anthracene modified single walled carbon nanotubes were deposited. The SWCNTs were in situ activated with a diazonium salt synthesized through the reaction of 2-amino anthracene with NaNO2 in acidic media (0.5 M HCl) for 5 min at 0 °C. After the in situ reaction, the 2-amino anthracene diazonium salt was electrodeposited by running cyclic voltammograms from +1000 mV to -1000 mV vs. Ag|AgClsat.