High-Throughput Nucleotide Sequencing

Recontructing paleoenvironments: Metabarcoding and shotgun sequencing Italian vulcanic lake sediments

Recontructing paleoenvironments: Metabarcoding and shotgun sequencing Italian vulcanic lake sediments

We are using the metabarcoding tecnique on sedimentary DNA (sedDNA) from three volcanic Italian lakes: Lago Grande di Monticchio, Lago di Nemi, and Lago di Martignano. At Monticchio, we have already identified over 100 plant taxa from a long sediment core covering the period from 1.4 to 30 thousand years before present. To maximize our results, we have developed new strategies to improve DNA extraction from mineral-adsorbed samples and counteract PCR inhibition.

PeachVar-DB: a curated collection of genetic variations for the interactive analysis of Peach Genome Data

Applying next-generation sequencing (NGS) technologies to species of agricultural interest has the potential to accelerate the understanding and exploration of genetic resources. The storage, availability and maintenance of huge quantities of NGS-generated data remains a major challenge. The PeachVar-DB portal, available at http://hpc-bioinformatics.cineca.it/peach, is an open-source catalog of genetic variants present in peach (Prunus persica L.

Minimal residual disease (MRD) in non-Hodgkin lymphomas: Interlaboratory reproducibility on marrow samples with very low levels of disease within the FIL (Fondazione Italiana Linfomi) MRD Network

In 2009, the four laboratories of the Fondazione Italiana Linfomi (FIL) minimal residual disease (MRD) Network started a collaborative effort to harmonize and standardize their methodologies at the national level, performing quality control (QC) rounds for follicular lymphoma (FL) and mantle cell lymphoma (MCL) MRD assessment.

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