Ricerc@Sapienza

Neutralophilic bacteria have developed several strategies to overcome the deleterious effects of acid stress. In particular, the amino acid-dependent systems are widespread, with their activities overlapping, covering a rather large pH range, from 6 to

The two most important and studied phytocannabinoids present in Cannabis sativa L. are undoubtedly cannabidiol (CBD), a non-psychotropic compound, but with other pharmacological properties, and Δ9-tetrahydrocannabinol (Δ9-THC), which instead possesses psychotropic activity and is responsible for the recreative use of hemp. Recently, the homolog series of both CBDs and THCs has been expanded by the isolation in a medicinal cannabis variety of four new phytocannabinoids possessing on the resorcinyl moiety a butyl-(in CBDB and Δ9-THCB) and a heptyl-(in CBDP and Δ9-THCP) aliphatic chain.

In this study ten organic apple varieties grown in Italy: Renetta Osiris, Gold Rush, Braeburn, Celato Cola, Limoncella, Cerina, Rosada, Topaz, Jonagored, Florina were analysed in order to evaluate some quality parameters. Individual phenolics compounds, total phenolics, glucose, fructose and antioxidant activity was determined in pulp and peel extracts. Results show that the chlorogenic acid was a predominant component in pulp and peel extracts, with highest value in Limoncella and Jonagored respectively.

Phenoxy acid herbicides are used worldwide and are potential contaminants of drinking water. Reversed phase high-performance liquid chromatography (RP-HPLC) is commonly used to monitor phenoxy acid herbicides in water samples. RP-HPLC retention of phenoxy acids is affected by both mobile phase composition and pH, but the synergic effect of these two factors, which is also dependent on the structure and pKa of solutes, cannot be easily predicted.

The quality of honey is influenced by botanical origins and acidity. 5-Hydroxymethyl-2-furaldehyde (HMF) is a well-monitored indicator for freshness and quality, whereas no restriction is reported for 2-furfural. The aim of the study is the determination of these compounds in 21 Italian honey samples through high-performance liquid chromatography (HPLC). In order to validate the HPLC method, the detection and quantification limits (LOD and LOQ, respectively), recovery, precision (repeatability), and linearity were determined.

An easy and effective analytical method was developed for the simultaneous quantification of four tocopherols
(Ts) and four tocotrienols (T3s) in three pecan nut cultivars (Stuart, Sioux, and Pawnee). The analytes were
separated on a C30 column kept at 15 °C, under isocratic non-aqueous reversed-phase (NARP) conditions, in only
18 min and detected by atmospheric pressure chemical ionization–tandem mass spectrometry (APCI-MS/MS).
The HPLC-APCI-MS/MS method was validated, according to the main FDA guidelines, and then applied for the

This review presents advancements in sample preparation and liquid chromatographic methods made during the last 10 years for the fat-soluble vitamin (FSV) analysis in foods. Since activity. and bioavailability of organic micronutrients (MOs) depend on the form in which they are present in a food, special emphasis has been given to the most recent techniques, instruments and approaches which have allowed the extraction and separation of various vitamin homologues as well as the comprehensive MO profiling in foods.

An undescribed labdane-like diterpene with a rare spiro-β-lactone function was identified from the ethanol
extract of the male cones of the coniferous tree Wollemia nobilis. This spirolabdadienolide (IUPAC name: syn-ent-
8(17),13-labdadien-19,18-olid-15-oic acid methyl ester; trivial name: wollemolide), was isolated by means of
traditional and high performance chromatography techniques and structurally elucidated through NMR and MS.
In addition, six further known metabolites were evidenced in the extract. Wollemolide, which may be considered

In this work the HPLC and NMR analysis of the phenyl-ethanoid glycosides (PhGs) pattern of a cultivated exemplar of Verbascum thapsus L. (Scrophulariaceae) from the Etnean area (Sicily, Italy) was performed in order to verify their possible presence. Wild V. thapsus is well-known in ethnopharmacology due to the several beneficial effects that it is able to exert and which are primarily due to these compounds. So, it’s extremely important that also cultivated exemplars of this species biosynthesize them in order to maintain their pharmacological properties.

Verbascum thapsus L., commonly known as great mullein or common mullein, is a biennial herbaceous plant belonging to the Scrophulariaceae family [1].
It has a long tradition of use in ethnopharmacology since it is well know to posses several medicinal properties going from the expectorant and demulcent to the diuretic, anti-inflammatory, sedative, astringent, antiviral, antibacterial and antifungal ones and for these it has been long used in several parts of the world to treat respiratory problems as well as earache, eczema, hemorrhoids, diarrhea, fever, tumor and allergies [2].