Ricerc@Sapienza

This paper evaluated the environmental impacts of different agronomic practices for a
hemp seed crop grown in Mediterranean environment. The following agricultural variables have been
considered: seven monoecious hemp varieties(Epsilon68 (E68), Fedora17 (F17), Felina32 (F32),
Ferimon (Fe), Futura75 (F75), Santhica27 (S27), Uso31 (U31)), three plant densities (40, 80,
and 120 plants m−2
), and two levels of nitrogen (N) fertilization (50 and 100 kg ha−1 of N). Life cycle

Cannabis oils, namely concentrated cannabis extracts, are getting plenty of attention because of their therapeutic potential for treatment of patients with cancer, HIV, multiple sclerosis and several other pathologies. Here we propose the use of ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) as alternative methods to the current protocols followed by pharmacists, the only authorized to manipulate standardized Cannabis. A third method, consisting of the use of Tween 20 as surfactant, was considered.