Unmatched kinetic performance in enantioselective supercritical fluid chromatography by combining latest generation whelk-O1 chiral stationary phases with a low-dispersion in-house modified equipment
This proof-of-concept work investigates the ultimate kinetic limits reachable in chiral supercritical fluid chromatography (SFC) with modern columns and advanced technological solutions. A commercial equipment (Waters Acquity UPC2) has been in-house modified to minimize its overall extra-column variance through a series of technical adjustments including low-volume connecting tubings, reduced-volume flow cell, an in-house made external column oven, external low-dispersion injection system, and electronic temperature controller.