The Fosso di Vallerano valley, Rome (Italy), is an alluvial valley characterized by a complex geological setting which implies significant vertical and horizontal heterogene- ities. In the last decade, this area experienced an intense urbanization which changes the ori- ginal seismic response conditions of free field. According to these features, the Fosso di Vallerano valley has been chosen as case study to analyze the influence of buildings on the local seismic response (Site-City Interaction – SCI).