On-chip LAMP-BART reaction for viral DNA real-time bioluminescence detection
The present paper describes the development of an integrated lab-on-chip, in which viral DNA amplifica- tion with real-time on-chip detection is carried out under constant temperature of 65 °C. The lab-on-chip is composed of a disposable 10-uL polydimethylsiloxane reaction chamber which is thermally and opti- cally coupled to a glass substrate that hosts a thin-film metallic resistive heater and thin-film amorphous silicon diodes which act as temperature and radiation sensors.