metabarcoding

Discovering symbiosis in the supralittoral: bacterial metabarcoding analysis from the hepatopancreas of Orchestia and Tylos (Crustacea)

Herein, we investigated for the first time the microbiota from the hepatopancreas of the talitrid amphipod Orchestia montagui and
the oniscidean isopod Tylos ponticus, two crustacean species associated with beached leaves of the seagrass Posidonia oceanica
(Posidonia banquette). We carried out a metabarcoding analysis based on several hypervariable regions of the bacterial 16S
rRNA gene. At the same time, three representative habitats of the supralittoral zone, namely leaf surface of Posidonia banquette,

Ancient plant DNA in lake sediments

Recent advances in sequencing technologies now permit the analyses of plant DNA from fossil samples (ancient plant DNA, plant aDNA), and thus enable the molecular reconstruction of palaeofloras. Hitherto, ancient frozen soils have proved excellent in preserving DNA molecules, and have thus been the most commonly used source of plant aDNA. However, DNA from soil mainly represents taxa growing a few metres from the sampling point.

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