Ricerc@Sapienza

Background/Aim: The identification of novel
prognostic biomarkers for melanoma metastasis is essential
to improve patient outcomes. To this aim, we characterized
miRNA expression profiles in relation to metastasis in
melanoma and correlated miRNAs expression with clinicalpathological factors. Materials and Methods: MiR-145-5p,
miR-150-5p, miR-182-5p, miR-203-3p, miR-205-5p and miR211-5p expression levels were analyzed in primary cutaneous
melanomas, including thin and thick melanomas, and in
melanoma metastases by quantitative Real-Time PCR.

Background: Melanoma cells develop adaptive responses in order to cope with particular conditions of tumor microenvironment, characterized by stress conditions and deregulated proliferation. Recently, the interplay between the stress response and the gene expression programs leading to metastatic spread has been reported. Methods: We evaluated levels and localization of eIF2α/peIF2α in V600BRAF and wtBRAF metastatic melanoma cell lines by means of western blot and confocal microscopy analyses.