Ricerc@Sapienza

Microalgae are an attractive source of metabolites, such as lipids, proteins, pigments and carbohydrates, of potential interest to the pharmaceutical, food and energy sectors. The aim of this study was to evaluate the effect of flocculation on lipid extraction from Chlorella Vulgaris. A 3 3 experimental design was performed with STATISTICA 7.0 software in order to determine the effects of flocculant (AlCl 3 ) addition, pH and time. The best strategy of pH adjustment-flocculant addition was evaluated, followed by lipid extraction under optimal conditions.

Both micro and macro algae have a potential to be a valuable feedstock for biorefineries. The theoretical impact assessment of this kind of plant can be carried out through an LCA, which is a key tool in order to evaluate the potential environmental impact of a process throughout its entire life cycle. Hence, it is a priority to perform an LCI with the aim of gathering all the data and simulating all the unit process of a theoretical biorefinery. The Inventory ensures to obtain a simple and immediate way to represent several aspects of a biorefinery, e.g.

A switchable-hydrophilicity solvent system, consisting of a fatty acid-based natural deep eutectic solvent
(NaDES), complemented by a bio-friendly dilute amine solution, has been introduced. The potential of
the most benign switchable solvent system has been characterised in microalgae biorefining according
to the recently proposed ‘Circular Extraction’ scheme.

In the last decade, interest toward the potential application of microalgae has grown considering their potential use in industrial sectors as human nutrition and health, animal feed and biopolymers. Their ability to use light or/and organic carbon as energy source, makes them able to grow in a wide range of conditions. Because of that, the possibility to use alternative nutrients and water sources for their cultivation has been investigated. The microalgal cultivation using wastewaters mixed with synthetic medium might be a good combination that could

This chapter covers sustainable techniques to perform a market-neutral biomass fractionation developed in laboratories, techniques targeting a substantial incorporation of the green chemistry principles, the paradigmatic shifts, and the role of green solvent technology in designing the future of biorefineries.

In a thin-volume photobioreactor where a concentrated suspension of microalgae is circulated throughout the established spatial irradiance gradient, microalgal cells experience a time-variable irradiance. Deploying this feature is the most convenient way of obtaining the so-called flashing light effect, improving biomass production in high irradiance. This work investigates the light flashing features of sloping wavy photobioreactors, a recently proposed type, by introducing and validating a computational fluid dynamics (CFD) model.