mixture design

Use of cell wall degrading enzymes to improve the recovery of lipids from Chlorella sorokiniana

An enzymatic treatment with cell wall degrading enzymes aimed at improving the recovery of lipids from Chlorella sorokiniana was investigated. Six commercial enzyme preparations containing cellulase, pectinase, lysozyme and three different hemicellulases were preliminary tested. The most effective preparations were then used as basic components for the formulation of enzyme cocktails with high cell wall degrading activity. Experiments were carried out according to the mixture design methodology.

Enhanced lycopene extraction from tomato peels by optimized mixed-polarity solvent mixtures

Mounting evidence from clinical and epidemiological studies suggests that lycopene, the most abundant carotenoid in tomatoes, may be beneficial in the prevention or treatment of some important diseases. Ripe tomato peels are the richest source of lycopene, but the use of conventional solvent extraction methods without pretreatment of the plant material results in very poor recovery. The reason lies in the localization of lycopene in the plant tissue and the low permeability of the latter to solvent molecules.

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