Ricerc@Sapienza

Introduction: The basis of the development of the anti-interleukin-5 monoclonal antibody mepolizumab was the acknowledgment of the crucial importance of this cytokine in promoting eosinophils

The immune system is known to play a pivotal role in cancer pathogenesis.
In a dynamic balance between immune system and cancer cells, the first one recognizes
the second as non-self and effectively clears them from the system. This phenomenon,
called immune surveillance, is based on the interaction between antigen
presenting cells and T lymphocytes that get activated eliciting a specific and enduring
response. In certain circumstances, tumor cells are able to evade this mechanism
allowing the tumor to develop. This mechanism is called tumor escape.

The aim of the present work has been the mass spectrometry characterization of the Nimotuzumab (NIM) antibody chemically modified with the bifunctional chelating agent para-S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraaza cyclododecanetetraacetic acid (p-SCN-Bn-DOTA).