In vivo non-radioactive assessment of mGlu5 receptor-activated polyphosphoinositide hydrolysis in response to systemic administration of a positive allosteric modulator.
mGlu5 receptor-mediated polyphosphoinositide (PI) hydrolysis is classically measured
by determining the amount of radioactivity incorporated in inositolmonophosphate
(InsP) after labeling of membrane phospholipids with radioactive inositol. Although this
method is historically linked to the study of mGlu receptors, it is inappropriate for the
assessment of mGlu5 receptor signaling in vivo. Using a new ELISA kit we showed
that systemic treatment with the selective positive allosteric modulator (PAM) of mGlu5