Necroptosis | Ricerc@Sapienza

RIP3 AND pMLKL promote necroptosis-induced inflammation and alter membrane permeability in intestinal epithelial cells

tBackground: Necroptosis is an inflammatory form of programmed cell death requiring receptor-interacting protein kinase 3 (RIP3) and mixed lineage kinase domain-like protein (MLKL).Aims: The aim of this study is to examine in depth in vitro and ex vivo the contribution of necroptosis tointestinal inflammation.Methods: In vitro: we used an intestinal cell line, HCT116RIP3, produced in our laboratory and overex-pressing RIP3.

Discovery, characterization and potential roles of a novel NF-YAx splice variant in human neuroblastoma

Background: Identification of novel cancer-associated splice variants is of potential diagnostic, prognostic and therapeutic importance. NF-Y transcription factor is comprised of NF-YA, NF-YB and NF-YC subunits, binds inverted CCAAT-boxes in ?70% of gene promoters, regulates > 1000 cancer-associated genes and proteins involved in proliferation, staminality, differentiation, apoptosis, metabolism and is subject to component alternative splicing.