nematode

In vitro and in vivo selection of potentially probiotic lactobacilli from Nocellara del Belice table olives

Table olives are increasingly recognized as a vehicle as well as a source of probiotic bacteria, especially those fermented with traditional procedures based on the activity of indigenous microbial consortia, originating from local environments. In the present study, we report characterization at the species level of 49 Lactic Acid Bacteria (LAB) strains deriving from Nocellara del Belice table olives fermented with the Spanish or Castelvetrano methods, recently isolated in our previous work. Ribosomal 16S DNA analysis allowed identification of 4 Enterococcus gallinarum, 3 E.

Caenorhabditis elegans and probiotics interactions from a prolongevity perspective

Probiotics exert beneficial effects on host health through different mechanisms of action, such as production of antimicrobial substances, competition with pathogens, enhancement of host mucosal barrier integrity and immunomodulation. In the context of ageing, which is characterized by several physiological alterations leading to a low grade inflammatory status called inflammageing, evidences suggest a potential prolongevity role of probiotics. Unraveling the mechanisms underlying anti-ageing effects requires the use of simple model systems.

The foodborne strain Lactobacillus fermentum MBC2 triggers pept-1-dependent pro-longevity effects in Caenorhabditis elegans

Lactic acid bacteria (LAB) are involved in several food fermentations and many of them
provide strain-specific health benefits. Herein, the probiotic potential of the foodborne strain
Lactobacillus fermentum MBC2 was investigated through in vitro and in vivo approaches.
Caenorhabditis elegans was used as an in vivo model to analyze pro-longevity and anti-aging effects.
L. fermentum MBC2 showed a high gut colonization capability compared to E. coli OP50 (OP50) or
L. rhamnosus GG (LGG). Moreover, analysis of pumping rate, lipofuscin accumulation, and body

Retargeting of a Plant Defense Protease by a Cyst Nematode Effector

Plants mount defense responses during pathogen attacks, and robust host defense suppression by pathogen effector proteins is essential for infection success. 4E02 is an effector of the sugar beet cyst nematode Heterodera schachtii. Arabidopsis thaliana lines expressing the effector coding sequence showed altered expression levels of defense response genes as well as higher susceptibility to both the biotroph H. schachtii and the necrotroph Botrytis cinerea, indicating a potential suppression of defenses by 4E02. Yeast two-hybrid analyses showed that 4E02 targets A.

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