Layer by layer supported laccase on lignin nanoparticles catalyzes the selective oxidation of alcohols to aldehydes
Lignin has been used as an electrochemical active nanostructured support for the immobilization of laccase from Trametes versicolor by encapsulation, physical adsorption, and layer by layer (LbL) technologies, including the glutaraldehyde and bovine derum albumin procedure. The activity parameters and kinetic properties of novel catalysts have been evaluated. Cyclic voltammetry and amperometric measurements confirmed the electrochemical behavior of the immobilized enzyme, which showed pseudo direct electron transfer (pseudo-DET) and mediated electron transfer (MET) mechanisms.