Ricerc@Sapienza

Optical biosensors based on one-dimensional photonic crystals sustaining Bloch surface waves are proposed to study antibody interactions and perform affinity studies. The presented approach utilizes two types of different antibodies anchored at the sensitive area of a photonic crystal-based biosensor. Such a strategy allows for creating two or more on-chip regions with different biochemical features as well as studying the binding kinetics of biomolecules in real time.

We report on hybrid inorganic/organic one-dimensional photonic crystal biochips sustaining Bloch surface waves. The biochips were used, together with an optical platform operating in a label-free and fluorescence configuration simultaneously, to detect the cancer biomarker Angiopoietin 2 in a protein base buffer. The hybrid photonic crystals embed in their geometry a thin functionalization poly-acrylic acid layer deposited by plasma polymerization, which is used to immobilize a monoclonal antibody for highly specific biological recognition.

We report on the use of an optical sensing platform based on Bloch surface waves sustained by one-dimensional photonic crystals as a novel optical tool to probe in real time the fluid flow at a boundarywall of a microfluidic channel under dynamic conditions. Understanding how fluid flow interacts withwall surfaces is crucial for a broad range of biological processes and engineering applications, such as sur-face wave biosensing. The proposed platform provides nanometric resolution with respect to the distancefrom the boundary wall sensor’s surface.