Comparison of 99m Tc-UBI 29-41, 99m Tc-ciprofloxacin, 99m Tc-ciprofloxacin dithiocarbamate and 111In-biotin for targeting experimental staphylococcus aureus and escherichia coli foreign-body infections. an ex-vivo study
BACKGROUND: Diagnosis of implant-associated infection is challenging. Several radiopharmaceuticals have been described but direct comparisons are limited. Here we compared in vitro and in an animal model Tc-99m-IJIBI, Tc-99m-cipmfloxacin, (TcN)-Tc-99m-CipmCS(2) and In-111-DTPA-biotin for targeting E. coli (ATCC 25922) and S. aureus (ATCC 43335).METHODS: Stability controls were performed with the labelled radiopharmaceuticals during 6 hours in saline and serum. The in vitro binding to viable or killed bacteria was evaluated at 37 degrees C and 4 degrees C.