SEM BSE 3D image analysis of human incus bone affected by cholesteatoma ascribes to osteoclasts the bone rrosion and VpSEM dEDX analysis reveals new bone formation
Bone erosion is considered a typical characteristic of advanced or complicated cholesteatoma (CHO), although it is still a matter of
debate if bone erosion is due to osteoclast action, being the specific literature controversial. The purpose of this study was to apply a
novel scanning characterization approach, the BSE 3D image analysis, to study the pathological erosion on the surface of human
incus bone involved by CHO, in order to definitely assess the eventual osteoclastic resorptive action. To do this, a comparison of