Ricerc@Sapienza

Interleukin-2 (IL2) is a cytokine that can stimulate cytotoxic immune cells to attack infected and malignant cells. Unfortunately, IL2 can also cause serious immune-related toxicity. Recently, a mutant of IL2 (IL2v) with abolished CD25 binding, increased plasma half-life and less toxicity was engineered. Unlike wild-type IL2 (wt-IL2), mutant IL2v does not bind to the ?-subunit (CD25) of the high affinity IL2??? receptor, but only to its ? and ? subunit. Here, we investigated the biological properties of IL2v and compared with the wt-IL2 using fluorine-18 and PET.

Oxidative stress and inflammation play a role in
the physiopathology of insulin resistance, diabetes and cardio-
vascular disease. A single high-fat, high-carbohydrate (HFHC)
meal induces an increase in inflammatory and oxidative
stress markers in peripheral blood mononuclear cells (PBMC).
Previous studies have shown that orange juice is able to prevent
this response by inhibiting toll like receptors (TLR) expression
and endotoxemia. Our goal was to study the proteome response
in PBMC after the consumption of a HFHC meal consumed