Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent
This paper reports the MEPS-HPLC-DAD method for the simultaneous determination of 12 azole drugs (bifonazole, butoconazole, clotrimazole, econazole, itraconazole, ketoconazole, miconazole, posaconazole, ravuconazole, terconazole, tioconazole and voriconazole) administered to treat different systemic and topical fungal infections, in biological samples. Azole drugs separation was performed in 36 min. The analytical method was validated in the ranges as follows: 0.02–5 ?g mL?1for ravuconazole; 0.2–5 ?g mL?1for terconazole; 0.05–5 ?g mL?1for the other compounds.