The ARP laboratory includes in detail the following equipment:
Applied Photophysics single-mixing -SX-18
Applied Photophysics Pi-star 180,
Applied Photophysics sequential-mixing DX-17MV
Tgk Scientific Hi-Tech PTJ-64 capacitor-discharge T-jump apparatus
The instrument is designed for high resolution mass spectrometry of proteins and nucleic acids.
The source can be operated in positive and negative ion mode using the linear or reflector detector. The saturation free detector technology enables highest dynamic range and superior performance for high-mass detection.
Animals respond to chemical stress with an array of gene families and pathways termed “chemical defensome”. In arthropods, despite many defensome genes have been detected, how their activation is arranged during toxic exposure remains poorly understood. Here, we sequenced the transcriptome of Anopheles stephensi larvae exposed for six, 24 and 48?hours to the LD50 dose of the insecticide permethrin to monitor transcriptional changes of defensome genes across time.
The newly synthesized coumarin derivative with dopamine, 3-(1-((3,4-dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione, was completely structurally characterized by X-ray crystallography. It was shown that several types of hydrogen bonds are present, which additionally stabilize the structure. The compound was tested in vitro against different cell lines, healthy human keratinocyte HaCaT, cervical squamous cell carcinoma SiHa, breast carcinoma MCF7, and hepatocellular carcinoma HepG2.
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