Ricerc@Sapienza

Highly hydrophilic and biocompatible nanocarriers based on polysaccharide hydrogels (nanohydrogels,
NHs) were shown to be promising systems for drug delivery applications. Following the idea of these
emerging drug carriers, the aim of the present work was to develop self-assembled hydrogel nanoparticles
based on amphiphilic derivatives of hyaluronic acid (HA) and riboflavin (Rfv), synthesized by “click”
Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC) reaction. The obtained amphiphilic product

Recent advances in biochemical and biophysical research have been achieved through the employment of microfluidic devices. Microfluidic mixing of therapeutic agents with biomaterials yields systems with finely tuned physical-chemical properties for applications in drug and gene delivery. Here, we investigate the role of preparation technology (microfluidic mixing vs. bulk self-assembly) on the transfection efficiency (TE) and cytotoxicity of multicomponent cationic liposome/DNA complexes (lipoplexes) in live Chinese hamster ovarian (CHO) cells.