A random positioning machine, or RPM, rotates biological samples along two independent axes to change their orientation in space in complex ways and so eliminate the effect of gravity. The RPM is a more sophisticated development of the single-axis clinostat. RPMs usually consist of two independently rotating frames.
Abstract: Changes in cell–matrix and cell-to-cell adhesion patterns are dramatically fostered by
the microgravity exposure of living cells. The modification of adhesion properties could promote
the emergence of a migrating and invasive phenotype. We previously demonstrated that short
exposure to the simulated microgravity of human keratinocytes (HaCaT) promotes an early epithelial–
mesenchymal transition (EMT). Herein, we developed this investigation to verify if the cells maintain
Microgravity is one of the most important features in space?ight. Previous evidence from in-vitro studies has shown that signi?cant changes occur under simulated microgravity. For thisreason, human colon adenocarcinoma Caco-2 cells were selected as cell model of intestinalepithelial barrier and their response to altered gravity conditions was investigated, especially onthe protein level.
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