Ricerc@Sapienza

This review presents advancements in sample preparation and liquid chromatographic methods made during the last 10 years for the fat-soluble vitamin (FSV) analysis in foods. Since activity. and bioavailability of organic micronutrients (MOs) depend on the form in which they are present in a food, special emphasis has been given to the most recent techniques, instruments and approaches which have allowed the extraction and separation of various vitamin homologues as well as the comprehensive MO profiling in foods.

This proof-of-concept work investigates the ultimate kinetic limits reachable in chiral supercritical fluid chromatography (SFC) with modern columns and advanced technological solutions. A commercial equipment (Waters Acquity UPC2) has been in-house modified to minimize its overall extra-column variance through a series of technical adjustments including low-volume connecting tubings, reduced-volume flow cell, an in-house made external column oven, external low-dispersion injection system, and electronic temperature controller.

The increasing interest for obtaining pure, single enantiomers of chiral organic compounds has greatly expanded the progress of new synthetic procedures, especially in the field of chiral drugs, agrochemicals, flavors, and natural products. A parallel trend has been observed in the development of stereoselective separation methods for the precise and accurate measurements of the enantiomeric purity of chiral compounds.