Background: Exercise-induced bronchoconstriction (EIB) reflects poor asthma control. Assessing noninvasive biomarkers associated with EIB could help to monitor patients in the pediatric age. Aims: To test exhaled and urinary biomarkers for assessing EIB in atopic asthmatic children.
Short peptide sequences represent emerging analytes in a variety of fields, including biomarker discovery, but also a well-known analytical challenge in complex matrices, due to the low abundance, extensive suppression during MS analysis, and lack of workflows, as they cannot be identified by ordinary peptidomics strategies and coverage is extremely limited by metabolomics as well.
The aims of the present studywere to establish reference values useful in monitoring Lithium(Li) treatment and
to trace environmental Li exposure profiles in paediatric age.
A cross-sectional studywas conducted on a group of healthy Italian children aged 5–11. Data on possible predictors
were assessed through a questionnaire, and Li levels in morning and evening urinary samples were determined
by ICP-MS technique.
The reference intervals for the evening and morning samples were respectively 3.8–51.9 μg L−1 or
The nuclear magnetic resonance (NMR)-based
metabolomic approach was used as analytical methodology
to study the urine samples of chronic inflammatory rheumatic
disease (CIRD) patients. The urine samples of CIRD patients
were compared to the ones of both healthy subjects and pa-
tients with multiple sclerosis (MS), another immuno-mediated
disease. Urine samples collected from 39 CIRD patients, 25
healthy subjects, and 26 MS patients were analyzed using
1
H
NMR spectroscopy, and the NMR spectra were examined
A pilot study was carried out on five obese/overweight patients suffering from metabolic syndrome, with the aim to evaluate postprandial effects of high fat/high glycemic load meals enriched by blueberries. Postprandial urine samples were analyzed by 1H-NMR spectroscopy after 2 and 4 h from ingestion to identify potential markers of blueberry intake. Significant decrease of methylamines, acetoacetate, acetone and succinate, known indicators of type 2 diabetes mellitus, were observed after the intake of meals enriched with blueberries.
A semi-quantitative method was developed to monitor the misuse of 15 SARM compounds belonging to nine different families, in urine matrices from a range of species (equine, canine, human, bovine and murine). SARM residues were extracted from urine (200 μL) with tert-butyl methyl ether (TBME) without further clean-up and analysed by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). A 12 min gradient separation was carried out on a Luna Omega Polar C18 column, employing water and methanol, both containing 0.1% acetic acid (v/v), as mobile phases.
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