Ricerc@Sapienza

Seizure-triggered maladaptive neural plasticity and neuroinflammation occur during the latent period as a key underlying event in epilepsy chronicization. Previously, we showed that α-tocopherol (α-T) reduces hippocampal neuroglial activation and neurodegeneration in the rat model of kainic acid (KA)-induced status epilepticus (SE). These findings allowed us to postulate an antiepileptogenic potential for α-T in hippocampal excitotoxicity, in line with clinical evidence showing that α-T improves seizure control in drug-resistant patients.

An easy and effective analytical method was developed for the simultaneous quantification of four tocopherols
(Ts) and four tocotrienols (T3s) in three pecan nut cultivars (Stuart, Sioux, and Pawnee). The analytes were
separated on a C30 column kept at 15 °C, under isocratic non-aqueous reversed-phase (NARP) conditions, in only
18 min and detected by atmospheric pressure chemical ionization–tandem mass spectrometry (APCI-MS/MS).
The HPLC-APCI-MS/MS method was validated, according to the main FDA guidelines, and then applied for the